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Enzyme kinetics: Determination of the Michaelis constant. P3050940

You can get the concept of:
The mechanism of Michaelis-Menton, the Law of proportions of chemical reactions, Principle Bodenseyna, electrolytic conductivity.

Principle:
We investigate the enzymatic hydrolysis of urea in aqueous solution, carbon dioxide and ammonia solution. Ions increase the conductivity of the urea solution. The rate of hydrolysis of urea by the enzyme urease is measured by monitoring the conductivity at different concentrations of the base, and is determined by the constant Michaelis-Menton.