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Enzyme kinetics: reduction of activity and poisoning of enzymes. P3051040

You can get the concept of:
Competitive, non-competitive blocking of the enzyme, reversible and irreversible blocking of the enzyme, catalysis, electrolytic conductivity.

Principle:
We investigate the enzymatic hydrolysis of urea in aqueous solution, carbon dioxide and ammonia solution. Ions increase the conductivity of the urea solution. By measuring the conductivity is determined by the rate of hydrolysis of urea at different concentrations of the base.