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Determination of Michaelis constant (with the chemical unit Kobra3). P4120340

You can get the concept of:
Michaelis constant, Enzymatic hydrolysis of urea, conductivity measurement, Principle Bodenstayna Complex grounds of the enzyme.

Principle:
Enzymatic hydrolysis of urea in aqueous solution releases carbon dioxide and ammonia. The ions of these compounds increase the conductivity of the solution. Conductivity measurement can be used to determine the rules of hydrolysis of urea by the enzyme urease at different concentrations. Michaelis constant can be calculated on the basis of these measurements.